PCR Reagents
The Appleton PCR range uses enhanced polymerases offering a range of uses from traditional amplification to high-fidelity cloning mixes. If you have a GC-rich or sample with PCR inhibitors our range has all the tools needed to easily optimise your work.
- Manufactured in the UK with the latest advances in anti-inhibitory technologies and optimised enzymes
- Our recombinant Taq DNA is highly purified and offers superior PCR performance for everyday tasks such as genotyping, screening and library construction
- Our range offers a wide variety of performance polymerases such as appMEGA for amplicons up to 35kb and works with GC and AT regions
appTAQ Polymerase & Ready-mixes
Reliable, every day PCR
- For routine PCR of amplicons up to 6kb
- Pre-optimised buffer system with pre-added dNTPs and MgCl2
- Works under standard or fast cycling conditions
- Clone PCR products into TA vectors
- Amplifies from complex GC-rich or AT-rich templates
appTAQ Hot Start Polymerase & Ready-mixes
Ultra sensitive PCR
- Inactive at ambient temperature for highly specific PCR
- For genotyping, high throughput PCR & low copy number detection
- Optimised buffer system with pre-added dNTPs and MgCl2
- Amplify products ≤6kb
- Suitable for difficult templates (low copy number, GC/AT-rich, blood, colony or methylated DNA)
- Works under standard or fast cycling conditions
- Clone PCR products into TA vectors
appHiFi Polymerase
Fast, Sensitive & High fidelity PCR
- 50 fold higher fidelity than appTAQ Polymerase
- Suitable for blunt end cloning, site-directed mutagenesis,
and next gen re-sequencing - Optimised buffer system with pre-added dNTPs and MgCl2
- Highly processive enzyme amplifies ≤10kb from complex GC- or AT- rich templates, colonies or crude samples
- Works under standard or fast cycling conditions
appMEGA Polymerase
For long range PCR of amplicons up to 35kb
- Hot-start enzyme is inactive at ambient temperature for highly specific PCR
- Optimised buffer system with pre-added dNTPs and MgCl2
- Works under standard or fast cycling conditions
- Clone PCR products into TA vectors
- Amplifies from complex GC- rich or AT- rich templates
- Suitable sample types: complex templates (GC- rich and AT- rich) / crude samples / multiplex / colony
Ordering Information
Downloads
appTAQ
| Code | Description | Units |
|---|---|---|
| ARP021 | appTAQ Hot Start Polymerase | 250 |
| ARP023 | appTAQ Hot Start Polymerase | 1,000 |
| ARP001 | appTAQ Polymerase | 500 |
| ARP003 | appTAQ Polymerase | 2,000 |
| ARP062 | appTAQ RedMix (2X), 200 reactions |
appHiFi
| Code | Description | Units |
|---|---|---|
| ARP041 | appHiFi Polymerase | 200 |
| ARP043 | appHiFi Polymerase | 1,000 |
appMEGA
| Code | Description | Units |
|---|---|---|
| ARP031 | appMEGA Polymerase | 250 |
| ARP033 | appMEGA Polymerase | 1,000 |
| ARP092 | appMEGA Mix (2X) |
Interested in our products? Find your nearest Appleton distributor
